LabrulezLCMS-EN

Quality decisions depend on more than instruments. Eurachem spotlights how pre- and post-analytical PT/EQA assess the full pathway—from sampling to interpretation—to drive quality and harmonisation. ISO 15189 stresses EQA should check the entire process, including pre- and post-examination.

• 🔍 Why it matters: reveals variation in sample prep, gaps vs guidelines, and inconsistent interpretation that routine PT can miss
• 🧪 Clinical chemistry: combine patient context + results; expert groups rank interpretative advice and share feedback
• 🧫 Microbiology (food poisoning): choose target organism(s), justify methods, report results, and decide on information dissemination
• 🧸 Toy safety: assess a sourced toy against EN 71; select clauses, tests, and labelling; compare with model answers
• 💧 Drinking water: evaluate near‑limit samples per Directive 98/83/EC; report results, uncertainty, and pass/fail; insights can inform regulators
• 🚀 Get started: find schemes via national accreditation bodies or EPTIS

Pre- and post-analytical proficiency testing Discover how pre- and post-analytical PT/EQA schemes improve the quality, harmonisation, and reliability of routine laboratory analyses.

Greener methods and the future of capillary LC headline the 44th International Symposium on Capillary Chromatography & 21st GCxGC Symposium 🌿🧪

• IUPAC Round Table: Greenness of official sample preparation methods 📅 Wed, May 20 🕐 13:00–14:00 📍 Room Dolomiti
AGREEprep review: most official methods score

Group Discussions at 44th ISCC & 21st GCxGC Discover key Group Discussions at ISCC & GCxGC 2026 covering green analytical chemistry, official methods, and the future of capillary LC adoption.

Separation science takes center stage at ISC 2026 🔬🧪🎯 The International Symposium on Chromatography (since 1956) presents high-impact tutorial lectures spanning chromatography, electrophoresis, mass spectrometry, miniaturized separations, and sample preparation.

• Tutorial faculty: Bezhan Chankvetadze (Tbilisi State University) 🧬; Martin Gilar (Waters Corporation) 💧; Václav Kašička (Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences) ⚗️
• Plus: Jörg P. Kutter (University of Copenhagen) 🧫; John A. McLean (Vanderbilt University) 🧪; Peter Schoenmakers (University of Amsterdam) 📚; František Švec (Faculty of Pharmacy, Charles University, Hradec Kralove) 🧪
• Program focus: pharma, environmental, food and health applications, with an exhibition and vendor seminars for the latest tools 🚀
• Who should attend: academia, industry, and government—prime networking and knowledge exchange 🤝
• Deadline alert: Early-bird registration and author payment close May 15, 2026 📅⏳

👉 Secure your spot now and power up your separation and detection strategies!

Tutorial Lectures at ISC 2026 Discover ISC 2026 tutorial lectures presented by leading scientists in chromatography, separation science, electrophoresis, and mass spectrometry.

Lights, camera, chromatography! 🎬🧪 HTC-19 invites you to the HTC Tube competition: show how your LC work impacts the world in a video up to 3 minutes—no pro gear needed. 📱

• Who: Scientists (academic or industrial) of any age across the LC workflow 👩‍🔬👨‍🔬
• What: Short, smartphone-friendly videos with real-world impact 🌍
• How: Electronic audience poll on scientific content, entertainment, creativity, and technical skill 🗳️✨
• Prizes: €750 🥇, €500 🥈, €250 🥉 (plus Belgian beer bragging rights) 🍺
• Deadline: Fri 15 May 2026 ⏰
• Submit: email your entry via the official HTC-19 event page 📩

Stand out at HTC-19—hit record and inspire the community! 🎥

HTC Tube Competition at the 19th HTC-19 Join the HTC-19 HTC Tube competition and submit a short video about your chromatography research for a chance to win prizes up to €750.

What happens when glycoproteomics meets real-time LC-MS decision making? 🚀
At the University of Washington, Nick Riley and the Riley Group are unlocking cell-surface biology using the Thermo Scientific Orbitrap Ascend MultiOmics Tribrid Mass Spectrometer paired with the Vanquish Neo UHPLC. 🔬⚙️

• Real-time library search powers Autonomous Dissociation Type Selection—choosing ETD, HCD, PTCR, and high-mass on the fly to maximize insight from a single injection. ⏱️🧠
• Flexible, reproducible separations reveal precious, low-abundance glycopeptides and intact glycoproteins. 🧪🎯
• Applied to protein corona studies with SEER nanoparticles despite double enrichment and scarce material. 🧬🧲
• Training-first mindset: robust tools democratize ETD so students chase big biological questions. 🎓💡
• Riley’s path: Josh Coon’s lab (University of Wisconsin-Madison) → Carolyn Bertozzi’s lab (Stanford), fueling a tools-first approach. 🛠️
• Kayla Markuson (Riley Group) received the 2025 Thermo Scientific Quantitative Proteomics Research Grant Program. 🏆

“Technology should expand the questions we can ask”—and real-time decision making is doing exactly that.

Glycoproteomics Meets Real-Time LC-MS Decision Making Orbitrap Ascend and Vanquish Neo enable flexible glycoproteomics workflows with ETD, HCD, and real-time LC-MS decision making.

🧬 A rare genetic disorder in the Amish community has illuminated a core cellular checkpoint in RNA splicing!

• CEITEC Masaryk University uncovered a precisely timed “go” signal: a chemical modification of SF3B1 that enables SNIP1 to bind and let splicing proceed. ✅🔬
• First author Pavla Gajdušková explains that without this signal, the cell stalls splicing—blocking correct gene processing. 🧠
• In affected Amish children, a tiny change in SNIP1 weakens this response, causing splicing errors that impair nervous system development (e.g., muscle weakness, seizures). ⚠️
• Led by Dalibor Blažek and team, the study confirms the checkpoint’s validity and shows how subtle protein changes can derail cellular function. ✨
• Beyond rare disease, the mechanism may also influence cell division—relevant to cancer research. 🎗️

Published in Nature Communications with collaborators from Spain and Germany. 🌍

Amish Disorder Confirms Key Cellular Mechanism Amish genetic disorder reveals RNA splicing checkpoint. Study by CEITEC Masaryk University clarifies a key cellular mechanism.

🧠🔬 New in J. Proteome Res.: CSF proteomics maps lysosomal and axonal disruptions in neurosyphilis
✍️ By Huayang Tang, Junjie Hu, Yifan Wu, Jinping Gao, Wenjun Wang, Xiaodong Zheng, Ran Zhang, Bo Liang, Fusheng Zhou, Ze Guo

• 🔬 Approach: LC–MS/MS discovery (28 individuals) + ELISA validation (110 samples) uncovered 234 differentially expressed CSF proteins
• 🧪 Pathways hit: lysosome (P = 0.007), axon guidance, neurodegeneration
• 📉 Key findings: Downregulated lysosomal proteins PSAP, DNASE2, CTSL, NPC2 and axonal protein EPHA4; strong PSAP–EPHA4 correlation (r = 0.83)
• ✅ Validation: Lower PSAP, DNASE2, CTSL, NPC2 confirmed in NS (n = 21) vs nNS (n = 56); ROC AUC—PSAP 0.91, DNASE2 0.95
• 🧬 Impact: Biomarker candidates and therapeutic clues; patterns align with signatures implicated in Alzheimer’s and Parkinson’s
• ⚠️ Limits: Cross-sectional design, limited ELISA panel; calls for longitudinal and mechanistic studies

🔗 Open access: https://doi.org/10.1021/acs.jproteome.5c00689

CSF Proteomics Reveals Neurodegeneration in Neurosyphilis CSF proteomics identifies lysosomal and axonal protein dysregulation linked to neurodegeneration in neurosyphilis.

🗓️ LabRulezLCMS Week 20/2026 is packed with HPLC and LC/MS insights! Elevate your methods with these power sessions:

• Thermo Fisher Scientific: Uncovering Microplastics—identification, quantification, exposure assessment 🔬🌍
• Waters Corporation: Size-variant analysis of antibodies and ADCs via SEC, SEC-MALS, SEC-MS, and 2D Protein A-SEC 🧬📈
• Waters Corporation: Beyond C18 and Phenyl—biphenyl columns for UV/pH robustness and metal-sensitive analytes 🧪⚖️
• Milestone: Modern sample prep for PAHs, PFAS, MOSH/MOAH in food with microwave-assisted extraction 🍽️⚡
• SelectScience: Faster GLP-1 amino acid analysis with UPLC, microwave hydrolysis, and Waters AccQ-Tag ⏱️💊
• Waters Corporation: Automate biomolecules workflows with Andrew+ and OneLab—consistency, traceability, efficiency 🤖✅

🔗 Details & registration: https://lcms.labrulez.com/webinars
🚀 Explore 5,500+ GC/LC/MS/spectroscopy webinars and filter what matters most.

Webinars LabRulezLCMS Week 20/2026 6 webinars: ADCs, automation, biomolecules, GLP-1, LC separations, microplastics, MOSH/MOAH, PFAS, SEC-MALS, SEC-MS, UPLC, workflow automation

A promising way to outsmart superbugs is here! 🧪🦠

• Led by Eva Kudová (IOCB Prague) and Jitka Viktorová (UCT Prague), researchers designed androstane-based steroid derivatives that turbocharge existing antibiotics. 🚀
• The best two molecules (compounds 13 and 16) block bacterial efflux pumps, slashing the doses needed for ciprofloxacin and erythromycin. 💊🔒
• One lead also suppresses Staphylococcus aureus virulence genes—hitting resistance and pathogenicity at once. 🧬🛡️
• Crucially, the most active compounds were non‑toxic to human immune cells and showed no detectable endocrine activity. ✅
• This positions steroidal scaffolds as potent antibiotic adjuvants to overcome efflux‑mediated resistance. 🔬

Authors: Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti‑Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová. Published in Eur. J. Med. Chem. (2026). 📄

Steroidal Hormones & Neurosteroids in Bacterial Therapy Androstane-based steroids can improve antibiotic efficacy against multidrug-resistant Staphylococcus aureus by targeting efflux-mediated resistance.

💊 New way to track metformin adherence in type 2 diabetes—straight from blood! 🔬

• What’s new: Untargeted metabolomics/lipidomics revealed N‑lactoyl‑amino acids (e.g., N‑lactoyl‑phenylalanine) as dose‑responsive plasma biomarkers of metformin exposure 🩸
• Why it matters: Objective, ultra‑sensitive (nanomolar) “fingerprint” that distinguishes regular vs. irregular use; levels rise up to 7.2× in high‑exposure patients 🎯
• How it was done: 637 metformin‑treated patients + 143 controls; 614 metabolites annotated; strong correlations with metformin (ρ = 0.42–0.55) 📈
• Extra insights: T2D shows elevated lactate, organic acids, BCAAs; lipid remodeling with more saturated TAGs/DAGs and fewer cholesteryl esters, sphingomyelins, phospholipids 🧬
• Who’s behind it: Tomáš Čajka, Laboratory of Translational Metabolism, Institute of Physiology CAS, with the Institute for Clinical and Experimental Medicine in Prague and the West Coast Metabolomics Center (University of California, Davis) 🤝
• Read more: Clin Pharmacol Ther 119:1057–1069 🔗 https://doi.org/10.1002/cpt.70205

New Biomarkers Track Metformin Adherence in Diabetes Advanced metabolomics revealed new plasma biomarkers that accurately monitor metformin adherence and metabolic response in type 2 diabetes patients.

EU food safety is tightening under Regulation 396/2005 — with 259 audits in 2025 and a default MRL of 0.01 mg/kg, labs have zero room for error. In a new article, David Oliva (Organomation) explains why precise QuEChERS evaporation is the make-or-break step.

• 🔬 What it means: broader scope, precautionary principle, annual control programs, complex residue definitions
• 🧪 The challenge: matrix effects, recoveries 70–120%, LC-MS/MS + GC-MS/MS coverage
• 💨 The bottleneck: concentrating 1–6 mL acetonitrile to 0.2–1 mL without loss, degradation, or contamination
• 🌬️ Why nitrogen blowdown wins: ambient-temp operation, volatility control, reproducible endpoints, clean pathways
• ⚙️ How N-EVAP helps: precise temperature control, individual needle flow, inert wetted parts, scalable 6–45 positions, audit-ready documentation
• ✅ Compliance impact: tighter LOQs (≤0.01 mg/kg), ISO/IEC 17025 workflows, stronger proficiency test performance

Ready to fortify your QuEChERS workflow for EU 396/2005? Organomation’s N-EVAP turns evaporation into a reliable, high-throughput control point for results that stand up to European Commission scrutiny.

EU Regulation 396/2005: Pesticide Residue Testing and the Critical Role of QuEChERS Evaporation in Food Safety Nitrogen concentration of QuEChERS extracts for reliable pesticide residue analysis under EU Regulation 396/2005.