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Microbial Analysis of Subgingival Plaque Samples Compared to That of Whole Saliva in Patients With Periodontitis

"The detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. The aim of this cross-sectional study is to investigate whether there is a correlation between the results of different bacterial species in saliva and subgingival plaque samples from individuals with aggressive periodontitis (AgP) and chronic periodontitis (CP). Whole saliva and subgingival plaque samples from the deepest pocket of each quadrant were collected from 43 patients with CP and 33 patients with AgP. Twenty different bacterial species from both samplings were determined by the 16S ribosomal RNA-based polymerase chain reaction with microarray technique. All bacterial species were detected in salivary and subgingival plaque samples. For Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, as well as Actinomyces viscosus, Campylobacter rectus/showae, Prevotella intermedia, Parvimonas micra, Eubacterium nodatum, and Campylobacter gracilis, a significant positive correlation between salivary and subgingival plaque samples was detected in patients with both types of periodontitis. There were no significant differences in bacteria in salivary and subgingival plaque samples between AgP and CP. Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP."

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PerioDX offers Superior Diagnostics and Targeted Treatments.

MicroGenDX is a molecular diagnostic laboratory that utilizes both Rapid qPCR Screening and Next-Generation DNA Sequencing (NGS) to identify all microbes within a sample taken from the site of infections. Our commitment to delivering the highest level of diagnostic data is why we utilize our 2-Part process of qPCR Rapid screening panel testing and NGS testing that uses our own curated database of 50,000+ bacterial and fungal microbial species.

Check out our website to keep up to date with all the latest dental scientific advances and information!

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💻 https://periodx.net/
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[email protected] for an introductory discount!

What Is The PerioDX Test Kit All About?

PerioDX tests saliva, periodontal pockets, and combines both for a single comprehensive microbial report.

📘 Definition Of The Day 📘
Endodontist is a dentist who practices or specializes in endodontics.

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PerioDX®—Precise Diagnosis Kit For Periodontal Diseases PerioDX® Is Now Available For Dentistry And Clinicians Focused On Treating Periodontal Diseases. Identify Over 50,000+ Microbes With 99.9% Accurate Detection!

Mean Annual Attachment, Bone Level, and Tooth Loss: A Systematic Review

"Characterizing participants at baseline by diagnosis, i.e., periodontitis and non-periodontitis is challenging. First, gingivitis and periodontitis are increasingly viewed as part of a continuum,1 and therefore an arbitrary threshold for diagnosis might lack validity. This is highlighted by the high prevalence values of at least mild forms of periodontitis which typically affect almost half of most populations.6-8 Similar difficulties exist with case definitions for other chronic conditions such as hypertension, diabetes, etc. For these conditions, case definitions are based on natural history/treatment studies, where subjects beyond a certain threshold have different health/treatment outcomes. As an analogy for periodontitis, a starting point might be to look across cohorts to determine whether there are subjects with a certain baseline periodontal status, who go on to lose more attachment and teeth and then define them as periodontally “healthy” or “severe.”
In addition to periodontal data, a consensus is required for a standardized data set of potential modifiers of attachment level change including certain oral microbiomes, genetic factors, lifestyle, general health, and socioeconomic measures.62
Finally, tooth loss, as a measure of periodontitis progression requires further research. Prevention of tooth loss is arguably the chief objective of prevention and treatment of periodontitis and is implicit in definitions of oral health.73 Although this parameter would potentially seem to be ideal in terms of being an objective measure and a true endpoint for assessing the impact of periodontal diseases,74 the many contributors to tooth loss/retention (e.g., patient preference, caries, dental professional treatment planning) complicate the interpretation of the data currently beyond very general observations. "

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PerioDX Is The Next Generation of Dental Diagnosis.

With our patient education bibliography, we allow patients and health professionals to easily stay “up-to-date” on Periodontal Disease and the Oral Systemic Connection.

OUR WEBSITE:
💻 https://periodx.net/
📧
For First Time Users, Email
[email protected] for an introductory discount!

PerioDX®—Precise Diagnosis Kit For Periodontal Diseases PerioDX® Is Now Available For Dentistry And Clinicians Focused On Treating Periodontal Diseases. Identify Over 50,000+ Microbes With 99.9% Accurate Detection!

The PerioDX test combines PCR and Next Generation Sequencing (NGS) to find what bacteria is there, vs. a limited panel of what a dentist or patient tests for. Our technology is the best in class for oral microbiome testing.

PerioDX Kit Includes:
• 2x 2mL Vials
• 1x 90mL Cup
• 1 BD 50 ML Syringe
• 2x 2mL 1x Bio Bag
• Lab Requisition
• Box With Return Label
• 1x 5mL Saline Solution

PerioDX®—Precise Diagnosis Kit For Periodontal Diseases PerioDX® Is Now Available For Dentistry And Clinicians Focused On Treating Periodontal Diseases. Identify Over 50,000+ Microbes With 99.9% Accurate Detection!

📘 Definition Of The Day 📘
Inflammation of gingival tissue without loss of connective tissue.

PerioDX®—Precise Diagnosis Kit For Periodontal Diseases PerioDX® Is Now Available For Dentistry And Clinicians Focused On Treating Periodontal Diseases. Identify Over 50,000+ Microbes With 99.9% Accurate Detection!

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It's Free Therapy 😁

PerioDX®—Precise Diagnosis Kit For Periodontal Diseases PerioDX® Is Now Available For Dentistry And Clinicians Focused On Treating Periodontal Diseases. Identify Over 50,000+ Microbes With 99.9% Accurate Detection!