Ellawe IVF

GYNEMED GM501 Wash is a ready-to-use medium formulated for washing human oocytes and embryos and for other short-term handling procedures outside of the incubator, including steps such as washing after hyaluronidase treatment (denudation), ICSI, polar body or blastomere biopsy, and similar manipulations.

Composition & Specifications

Contains salts (NaCl, KCl, KH₂PO₄, MgSO₄·7H₂O, CaCl₂·2H₂O), NaHCO₃, glucose, lactate, pyruvate, EDTA, Alanyl-Glutamine, amino acids, HEPES (15 mM), and human serum albumin at 5.00 g/L.

pH (37 °C, 6 % CO₂): 7.20 – 7.50

Osmolality: 270 – 290 mOsm/kg

Sterility: Sterile, SAL = 10⁻³ (Sterility Assurance Level)

Endotoxin: < 0.25 EU/mL

MEA performance: ≥ 80 % of embryos reach blastocyst stage after 96 h (with 60 min exposure at zygote stage)

Usage Instructions

The medium should be equilibrated overnight in a humidified CO₂ incubator (5–7 % CO₂, 37 °C) before use.

For oocyte/embryo washing: prepare dishes with drops (15–100 µL) or larger volumes (0.5–1 mL) of GM501 Wash under oil, then pass oocytes/embryos through several droplets of the medium, following the lab’s protocol.

The medium can also be used for micromanipulation steps like assisted hatching, biopsy, etc., outside the incubator.

Storage & Stability

Shelf life: 6 months from manufacture.

Storage: 2-8 °C, do not freeze.

After opening: usable for up to 7 days if sterile conditions are maintained and stored at 2-8 °C.

GYNEMED GM501 Wash 50ml A ready-to-use medium for washing human oocytes and embryos, and for short-term manipulation steps outside the incubator.

position push-on cap that allows for venting and helps prevent sample loss or fluid evaporation. Manufactured from medical-grade materials (such as Polystyrene, Polypropylene, or Polyethylene as per specification), sterilized via gamma irradiation, with a Sterility Assurance Level of 10⁻⁶, and CE marked according to 98/79/EC for in vitro diagnostic devices. Each lot is tested for:
• One-cell stage mouse embryo assay (MEA ≥ 80% blastocyst rate)

• Endotoxin level (LAL ≤ 0.03 EU/mL)

14 mL OPU Tube OT10 A 14 mL OPU tube with a push-on cap, sterile, for safely collecting oocytes in IVF procedures.

The Sarstedt 70.3010.305 pipette tip is intended for precise liquid handling of small volumes (10 µL). Made from high-purity polypropylene (PP), it is transparent and has filling level rings for accurate volume reading. The product is supplied in a SingleRefill pack of 96 tips.
It belongs to the In-Vitro Diagnostic (IVD) category, meets required safety and sterilization standards. It is autoclavable at up to 121 °C. It does not include a filter. The colour coding is grey.

Sarstedt Single Refill10 µL Pipette Tip Transparent 10 µL pipette tip with graduation marks, Biosphere® plus purity, from Sarstedt. Comes in a 96-piece SingleRefill pack.

https://ellawe.com/en/products/ot10

14 mL OPU Tube OT10 A 14 mL OPU tube with a push-on cap, sterile, for safely collecting oocytes in IVF procedures.