Medical Laboratory Science -MLS

What are the clinical significance of Albumin?

Albumin is the major constituent of plasma protein (60%) which consist of single polypeptide chain of 585 amino acids with 17 disulphide bond.

What's the Normal conc of Albumin?

Plasma protein is a blood component that contains anticoagulants and activated protein factors

What are the components of plasma protein?

For every 1 patient a doctor sees, at least 3 Lab tests are required. If a doctor says he attended to 100 patients and is overworked, meaning a MLS is working on over 300 tests. Now tell me why they are being selfish and want to peg other HCW with a stupid salary ratio?

Bacteria multiply faster than your weekends break

Shigellosis are gram negative rods, like other enterobacteriacae, found in human intestinal tract as pathogens.

What are the various species of shigella❓

HAEMOPHILIUS

Haemophilius are gram negative, cocco baccili, they can assume different shapes. The genius causes different infections. The Haemophilius influenza is the most virulent among, causing bloodstream infection and meningitis in children younger than 2 years.

What are the various species of the genus Haemophilius❓

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🦠 Gram Staining: A Simple Technique 🧪

Gram Staining process, a laboratory technique used to classify bacteria as either Gram-positive or Gram-negative based on their cell wall structure.
The steps are:
1. Crystal Violet (Purple): Applied for 60 seconds to stain all cells purple.

2. Iodine (Orange): Added for 60 seconds as a mordant to fix the purple stain to the cell wall.

3.95% Ethyl Alcohol: Used for 5-10 seconds to decolorize Gram-negative bacteria, while Gram-positive bacteria retain the purple color.

4.Safranin (Pink): Applied for 45 seconds to stain Gram-negative bacteria pink, leaving Gram-positive bacteria purple.

Final result:
Gram-positive bacteria appear purple.
Gram-negative bacteria appear pink.

This method is essential for diagnosing bacterial infections.