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noticed In these analyses, co-positivity best distinguished neoantigen-specific benefit using blockade as monotherapy in Brammer et al, O'Reilly et al. However, we also previously

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mirrors a subset of that in humans, suggesting that these preclinical models accurately recapitulate a subset of human disease. Elevated expression in mice and humans Given the

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"classify" and refine by computing cells and examined expression of genes encoding for cell depletion in these selected cells as well as expression of gene signatures of the source

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observed population profiles in most tumors, suggesting the presence of recently activated proliferating and non-senescent intratumoral cells with potential for therapeutic rejuvenation.

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dysfunction in the context of this disease. Humans contain a similarly depleted intratumoral cell population To investigate whether these observations could be extended to humans, we

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tumors. Progenitor cell-like has been shown Evidence is the basis for the proliferative burst in the blocking response Miller et al., Siddiqui et al. What we observed in most tumors, but

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isolated intratumoral cells from freshly resected surgical samples for flow cytometry analysis. Of the samples evaluated, 4 had sufficient range for further immunophenotyping. Consistent

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Chihara et al., Schittinger et al., we examined co-inhibitory receptor expression on observed clusters had higher gene expression profiles associated with cellular depletion. Then we

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